protogel 009Use ProtoGel Stacking Buffer to make 10 mL of a 4% stacking gel as follows: ProtoGel: 1.33 mL ProtoGel Stacking Buffer: 2.50 mL Deionized Water: 6.11 mL Afterwards, add 0.05 mLProtoGel is a stabilized, ready-to-use 30% (wv) acrylamidemethylene bisacrylamide solution (37.5:1 ratio) manufactured from the hhest quality materials from which virtually all impurities