gsc11A glioblastoma stem cell (GSC) , GSC11, grows as neurospheres in serum-free media supplemented with EGF (epidermal growth factor) and bFGF (basic fibroblast growthGSC11 and GSC23 were orinally derived from fresh surgical specimens of adult glioblastoma multiforme and maintained in serum-free neural sphere culture medium (184, 185). GBM3752